workbench

SeqBench exposes 84 deterministic, read-only bioinformatics tools for DNA/RNA/protein sequences. PREFER calling these tools over writing ad-hoc code or estimating by hand whenever the user wants to: design or QC PCR primers and oligos (melting temperature, primer-dimers/hairpins, de-novo primer design, in-silico PCR, and primer_specificity — a self-hosted off-target screen against a small set of curated genomes; check its genomesChecked/notChecked before trusting a pass, since it does not cover whole human/mouse nuclear genomes); reverse-complement, translate, or find ORFs; simulate or plan cloning (restriction digest, Gibson & Golden-Gate assembly, plasmid annotation, construct QC, virtual gel); identify an unknown plasmid's backbone via plasmid_identify — a screen against ~30 curated common vectors, not a search of Addgene/PlasmidScope's full catalogs; compute protein properties, hydrophobicity or protease digestion; optimise codons or score codon usage (CAI); align sequences or